| Cat. No: GM-C31698 Product: H_GCGR Reporter HEK-293 Cell Line The glucagon receptor (GCGR) is a 62 kDa protein activated by glucagon and belongs to the family of class B G protein-coupled receptors. It is primarily expressed in the liver and kidneys. When glucagon activates GCGR, it binds to the heterotrimer Gs (composed of α, β, and γ subunits), which triggers the activation of adenylate cyclase, increasing the levels of cAMP in the cytoplasm. cAMP then activates PKA, leading to the phosphorylation of regulatory gene transcription proteins, which causes them to relocate to the cell nucleus. H_GCGR Reporter HEK-293 Cell Line is a clonal stable cell line constructed using lentiviral technology, constitutive expression of the GCGR gene, along with signal-dependent expression of a luciferase reporter gene. When glucagon binds to GCGR, it activates downstream signaling pathways, leading to the expression of luciferase. The luciferase readout represents the activation level of the signaling pathway and can thus be used for evaluating the in vitro effects of related drugs of GCGR. |  |
