| Cat. No: GM-C19979 Product: H_CALCRL RAMP1 Reporter HEK-293 Cell Line CGRP family receptors are classified into two subtypes: the calcitonin receptor (CALCR, CTR) that binds CT and the calcitonin receptor-like receptor (CRLR, CLR, CALCRL). The CGRP receptor comprises the calcitonin receptor-like receptor, receptor activity-modifying protein 1 (RAMP1), and CGRP receptor component protein (CRCP). RAMP1 is crucial for transporting the receptor to the plasma membrane and coupling it with CGRP agonists, while CRCP connects the CGRP receptor to G proteins. Upon ligand binding, the complex interacts with G proteins, leading to the dissociation of the Gαs subunit, which activates adenylate cyclase and produces cAMP. The rise in intracellular cAMP stimulates signaling pathways, resulting in the binding of the transcription factor cAMP response element-binding protein to the promoter and inducing gene expression. H_CALCRL RAMP1 Reporter HEK-293 Cell Line is a clonal stable HEK-293 cell line constructed using lentiviral technology, constitutive expression of human CALCRL, human RAMP1, along with cAMP signal-dependent expression of a luciferase reporter gene. When α-CGRP/β-CGRP binds to CALCRL/RAMP1, it activates downstream signaling pathways, leading to the expression of luciferase. antagonists can inhibit this signal transmission. The luciferase activity measurement indicates the activation level of the signaling pathway and can thus be used to evaluate the in vitro effects of drugs related to CALCRL/RAMP1. |  |
