细胞生长培养基：DMEM+10% FBS+1% P.S+2.5 μg/mL Puromycin+2 μg/mL Blasticidin+200 μg/mL Hygromycin
细胞冻存液：90% FBS+10% DMSO
细胞生长培养基：DMEM+10% FBS+1% P.S+2.5 μg/mL Puromycin+2 μg/mL Blasticidin+200 μg/mL Hygromycin
细胞冻存液：90% FBS+10% DMSO
基于MC38细胞，使用慢病毒技术构建的稳定表达H_PDL1的单克隆细胞系。
H_PDL1氨基酸序列(NP_054862.1)：
MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET
细胞生长培养基：DMEM+10% FBS+1% P.S+2.5 μg/mL Puromycin+2 μg/mL Blasticidin+200 μg/mL Hygromycin
细胞冻存液：90% FBS+10% DMSO
细胞生长培养基：DMEM+10% FBS+1% P.S+2.5 μg/mL Puromycin+2 μg/mL Blasticidin+200 μg/mL Hygromycin
细胞冻存液：90% FBS+10% DMSO
基于MC38细胞，使用慢病毒技术构建的稳定表达H_PDL1的单克隆细胞系。
H_PDL1氨基酸序列(NP_054862.1)：
MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET
基于MC38细胞，使用慢病毒技术构建的稳定表达H_PDL1的单克隆细胞系。
H_PDL1氨基酸序列(NP_054862.1)：
MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET
