生长培养基：DMEM+10% FBS+1% P.S+10 μg/mL Blasticidin+0.5 μg/mL Puromycin
冻存培养基：90% FBS+10% DMSO
Assay Buffer：DMEM+1% FBS+1% P.S
生长培养基：DMEM+10% FBS+1% P.S+10 μg/mL Blasticidin+0.5 μg/mL Puromycin
冻存培养基：90% FBS+10% DMSO
Assay Buffer：DMEM+1% FBS+1% P.S
基于B16-F10细胞，使用慢病毒技术构建的稳定表达H_MICA*001的单克隆细胞系。
H_MICA*001氨基酸序列
MGLGPVFLLLAGIFPFAPPGAAAEPHSLRYNLTVLSWDGSVQSGFLTEVHLDGQPFLRCDRQKCRAKPQGQWAEDVLGNKTWDRETRDLTGNGKDLRMTLAHIKDQKEGLHSLQEIRVCEIHEDNSTRSSQHFYYDGELFLSQNLETKEWTMPQSSRAQTLAMNVRNFLKEDAMKTKTHYHAMHADCLQELRRYLKSGVVLRRTVPPMVNVTRSEASEGNITVTCRASGFYPWNITLSWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRICQGEEQRFTCYMEHSGNHSTHPVPSGKVLVLQSHWQTFHVSAVAAAAIFVIIIFYVRCCKKKTSAAEGPELVSLQVLDQHPVGTSDHRDATQLGFQPLMSDLGSTGSTEGA
生长培养基：DMEM+10% FBS+1% P.S+10 μg/mL Blasticidin+0.5 μg/mL Puromycin
冻存培养基：90% FBS+10% DMSO
Assay Buffer：DMEM+1% FBS+1% P.S
基于B16-F10细胞，使用慢病毒技术构建的稳定表达H_MICA*001的单克隆细胞系。
H_MICA*001氨基酸序列
MGLGPVFLLLAGIFPFAPPGAAAEPHSLRYNLTVLSWDGSVQSGFLTEVHLDGQPFLRCDRQKCRAKPQGQWAEDVLGNKTWDRETRDLTGNGKDLRMTLAHIKDQKEGLHSLQEIRVCEIHEDNSTRSSQHFYYDGELFLSQNLETKEWTMPQSSRAQTLAMNVRNFLKEDAMKTKTHYHAMHADCLQELRRYLKSGVVLRRTVPPMVNVTRSEASEGNITVTCRASGFYPWNITLSWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRICQGEEQRFTCYMEHSGNHSTHPVPSGKVLVLQSHWQTFHVSAVAAAAIFVIIIFYVRCCKKKTSAAEGPELVSLQVLDQHPVGTSDHRDATQLGFQPLMSDLGSTGSTEGA
